在固氮蓝细菌中编码丙酮酸：铁氧化还原蛋白氧化还原酶的nifJ基因的鉴定。
Identification of the nifJ gene coding for pyruvate: ferredoxin oxidoreductase in dinitrogen-fixing cyanobacteria.
作者信息
Schmitz O, Kentemich T, Zimmer W, Hundeshagen B, Bothe H
机构信息
Botanisches Institut, Universität zu Köln, Germany.
出版信息
Arch Microbiol. 1993;160(1):62-7.
A 329 bp DNA segment from both Anabaena variabilis and Anabaena PCC 7119 was amplified using the polymerase chain reaction (PCR). The sequences from the two cyanobacteria showed strong similarities to the corresponding part of the nifJ gene from Klebsiella pneumoniae and Enterobacter agglomerans. The present findings underline earlier results of enzymatic studies that heterocystous cyanobacteria possess a pyruvate:ferredoxin (flavodoxin) oxidoreductase (PFO). The nifJ gene segment could not be detected in the non-dinitrogen-fixing, unicellular cyanobacterium Anacystis nidulans which is also in accord with previous findings from enzyme assays.
利用聚合酶链反应（PCR）扩增了可变鱼腥藻和鱼腥藻PCC 7119的一段329 bp DNA片段。这两种蓝细菌的序列与肺炎克雷伯菌和聚团肠杆菌nifJ基因的相应部分具有很强的相似性。目前的研究结果强调了早期酶学研究的结果，即异形胞蓝细菌拥有丙酮酸：铁氧化还原蛋白（黄素氧化还原蛋白）氧化还原酶（PFO）。在不固氮的单细胞蓝细菌集胞藻中未检测到nifJ基因片段，这也与之前酶分析的结果一致。
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