嗜热栖热放线菌中编码碳水化合物底物降解酶的基因在大肠杆菌中的克隆与表达
Cloning and expression in Escherichia coli of Thermotoga neapolitana genes coding for enzymes of carbohydrate substrate degradation.
作者信息
Dakhova O N, Kurepina N E, Zverlov V V, Svetlichnyi V A, Velikodvorskaya G A
机构信息
Institute of Molecular Genetics, Russian Academy of Science, Moscow.
出版信息
Biochem Biophys Res Commun. 1993 Aug 16;194(3):1359-64. doi: 10.1006/bbrc.1993.1974.
A genomic library of thermophilic anaerobic eubacterium Thermatoga neapolitana was constructed in E. coli using the pTZ19R plasmid vector. Some groups of recombinant clones with different cellulase activities were revealed: clones carrying genes for an 1,4-beta-glucanases, 1,3-beta-glucanases, beta-xylanases, beta-glucosidases and beta-xylosidases. One clone possessing avicelase activity was obtained. Some clones were selected with amylolytic activities toward amylose, amylopectin and pullulan.
使用pTZ19R质粒载体在大肠杆菌中构建了嗜热厌氧真细菌那不勒斯栖热袍菌的基因组文库。发现了一些具有不同纤维素酶活性的重组克隆组：携带1,4-β-葡聚糖酶、1,3-β-葡聚糖酶、β-木聚糖酶、β-葡萄糖苷酶和β-木糖苷酶基因的克隆。获得了一个具有微晶纤维素酶活性的克隆。选择了一些对直链淀粉、支链淀粉和支链淀粉酶具有淀粉分解活性的克隆。
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