Poumay Y, Boucher F, Leclercq-Smekens M, Degen A, Leloup R
Département d'Histologie-Embryologie, Facultés Universitaires Notre-Dame de la Paix, Namur, Belgium.
Epithelial Cell Biol. 1993 Jan;2(1):7-16.
The contribution of adhesion to an extracellular matrix in the polarized spatial organization of keratinocytes was studied in dispase-detached cultures stored as floating sheets. Proliferating and terminally differentiating cell populations were, therefore, localized on tissue sections by their DNA-synthesizing ability and involucrin immunostaining, respectively. A progressive reorganization was induced from superposed proliferating and differentiating layers into clusters exhibiting differentiating cells on the outside. Measurements of proliferation and terminal differentiation in detached cultures revealed the progressive disappearance of proliferating cells, followed by an increase in involucrin-positive cells. Attempts to block the spatial reorganization by the addition of components of the extracellular matrix remained unsuccessful. These results suggest that basal anchorage is responsible for the superposition of proliferating and differentiating cells in keratinocyte cultures. They afford new arguments for the induction of terminal differentiation in non-adhesive keratinocytes which exhibit a concomitant modification of cell shape.
在作为漂浮片保存的分散酶分离培养物中，研究了黏附于细胞外基质对角质形成细胞极化空间组织的作用。因此，通过DNA合成能力和内披蛋白免疫染色，分别在组织切片上定位增殖细胞群和终末分化细胞群。从叠加的增殖层和分化层诱导出渐进性重组，形成外部为分化细胞的细胞簇。对分离培养物中的增殖和终末分化进行测量，结果显示增殖细胞逐渐消失，随后内披蛋白阳性细胞增加。通过添加细胞外基质成分来阻断空间重组的尝试未成功。这些结果表明，基底锚定负责角质形成细胞培养物中增殖细胞和分化细胞的叠加。它们为非黏附性角质形成细胞中终末分化的诱导提供了新的论据，这些细胞同时伴随着细胞形状的改变。
