Represa A, Jorquera I, Le Gal La Salle G, Ben-Ari Y
INSERM, U29, Paris, France.
Hippocampus. 1993 Jul;3(3):257-68. doi: 10.1002/hipo.450030303.
In the present study, using Golgi and electron microscopy techniques, experimentally induced epilepsy (kindling and kainate treatment) elicited collateral sprouting of mossy fibers in rat hippocampus. Collateral branches invade the hilus, cross the granule cell layer, and distribute throughout the inner third of the molecular layer. These newly developed collaterals may acquire the typical features of mossy fibers including giant fiber varicosities (mousses), although the mean surface of these mousses was thinner in these collaterals than in terminal branches. Granule cell dendrites may develop giant thorny excrescences, suggesting that the targets of these collaterals are granule cells. Giant synaptic boutons appear in the inner third of molecular layer of epileptic rats. These boutons acquire the morphological features of mossy fiber boutons and made multiple synaptic contacts with dendritic spines. The analysis of the profile types suggests that some of the newly developed collateral mossy fibers made hypotrophic synaptic contacts.
在本研究中，运用高尔基染色法和电子显微镜技术，实验性诱导的癫痫（点燃和 kainate 处理）引发了大鼠海马体中苔藓纤维的侧支发芽。侧支侵入齿状回，穿过颗粒细胞层，并分布于分子层内三分之一区域。这些新形成的侧支可能获得苔藓纤维的典型特征，包括巨大的纤维膨体（苔藓球），尽管这些苔藓球在这些侧支中的平均表面积比在终末分支中更薄。颗粒细胞树突可能会形成巨大的棘状赘生物，这表明这些侧支的靶细胞是颗粒细胞。巨大的突触小体出现在癫痫大鼠分子层的内三分之一区域。这些突触小体获得了苔藓纤维突触小体的形态特征，并与树突棘形成多个突触联系。轮廓类型分析表明，一些新形成的侧支苔藓纤维形成了发育不良的突触联系。
