Boerboom L E, Olinger G N, Karas B J, Lindon J N, Rosen L Z, Thyagarajan K, Tu R
Department of Cardiothoracic Surgery, Medical College of Wisconsin, Milwaukee.
Int J Artif Organs. 1993 May;16(5):263-7.
An alternative graft is needed for coronary bypass operations in patients lacking suitable autologous vessels. We therefore studied Denaflex, a biologic graft, in a dog ex-vivo shunt model to determine whether heparin treatment makes this graft less thrombogenic. Comparison was also made to Bioflow, a nonheparinized biologic graft. Fibrinogen deposition during high flow (593 +/- 202 ml/min) decreased from 672 +/- 467 ng/mm2 in nonheparinized Denaflex grafts to 448 +/- 298 ng/mm2 (p < 0.05) in heparinized Denaflex grafts. At low flow (117 +/- 13 ml/min), heparinization of Denaflex grafts similarly decreased fibrinogen deposition from 1102 +/- 601 ng/mm2 to 703 +/- 405 ng/mm2 (p < 0.05). At both flow rates fibrinogen deposition in Bioflow grafts was less than in nonheparinized Denaflex, but was similar to heparinized Denaflex grafts. Platelet deposition was not influenced by heparinization of Denaflex grafts and was similar among Denaflex and Bioflow preparations. Whether Denaflex performs acceptably in vivo as a xenograft requires extensive study.
对于缺乏合适自体血管的患者，冠状动脉搭桥手术需要替代移植物。因此，我们在犬体外分流模型中研究了生物移植物Denaflex，以确定肝素治疗是否能降低该移植物的血栓形成倾向。同时也与未用肝素处理的生物移植物Bioflow进行了比较。在高流量（593±202毫升/分钟）时，未用肝素处理的Denaflex移植物中纤维蛋白原沉积从672±467纳克/平方毫米降至肝素处理的Denaflex移植物中的448±298纳克/平方毫米（p<0.05）。在低流量（117±13毫升/分钟）时，Denaflex移植物的肝素化同样使纤维蛋白原沉积从1102±601纳克/平方毫米降至703±405纳克/平方毫米（p<0.05）。在两种流速下，Bioflow移植物中的纤维蛋白原沉积均少于未用肝素处理的Denaflex移植物，但与肝素处理的Denaflex移植物相似。Denaflex作为异种移植物在体内的表现是否可接受需要进行广泛研究。
