Kinoshita M, Ross A C
Department of Biochemistry, Medical College of Pennsylvania, Philadelphia 19129.
J Immunoassay. 1993 Sep;14(3):149-66. doi: 10.1080/15321819308019846.
Quantitative ELISAs have been developed for the four immunoglobulin G (IgG) subclasses (IgG1, IgG2a, IgG2b and IgG2c) of the rat. These assays were shown to have excellent sensitivity, reproducibility and adequate specificity for studies of natural IgG and antigen-elicited IgG responses. The sensitivity (working range for each isotype assay) was: IgG1, 1.6 to 200 ng/ml; IgG2a, 8 to 200 ng/ml; IgG2b, 1.6 to 200 ng/ml; and IgG2c, 1.6 to 1000 ng/ml. The isotype sum and the direct assay of total plasma IgG agreed closely. The utility of these assays was demonstrated in a study of the development of total IgG and of the specific IgG response following immunization with tetanus toxoid (TT). For total IgG, the predominant isotype was IgG2b (64% of total IgG) followed by IgG2a (29%), IgG1 (4%) and IgG2c (3%). In contrast, the anti-TT response was mainly of the IgG1 subclass (57% of total anti-TT). These quantitative assays should prove useful for investigating the response to experimental vaccines and the influence of cytokines on class switching in vivo.
已开发出针对大鼠四种免疫球蛋白G（IgG）亚类（IgG1、IgG2a、IgG2b和IgG2c）的定量酶联免疫吸附测定（ELISA）。这些测定方法在研究天然IgG和抗原引发的IgG反应方面显示出极佳的灵敏度、重现性和足够的特异性。其灵敏度（每种同型测定的工作范围）为：IgG1，1.6至200 ng/ml；IgG2a，8至200 ng/ml；IgG2b，1.6至200 ng/ml；IgG2c，1.6至1000 ng/ml。同型总和与血浆总IgG的直接测定结果密切相符。在用破伤风类毒素（TT）免疫后对总IgG的发育以及特异性IgG反应的研究中证明了这些测定方法的实用性。对于总IgG，主要同型为IgG2b（占总IgG的64%），其次是IgG2a（29%）、IgG1（4%）和IgG2c（3%）。相比之下，抗TT反应主要为IgG1亚类（占总抗TT的57%）。这些定量测定方法应被证明对研究实验性疫苗的反应以及细胞因子对体内类别转换的影响有用。
