Akao N, Goto Y, Kondo K, Tsuda Y
Department of Parasitology, School of Medicine, Kanazawa University, Japan.
J Helminthol. 1993 Jun;67(2):145-50. doi: 10.1017/s0022149x00013031.
Second-stage larvae of Toxocara canis were maintained in vitro for one year. Susceptibility of the larvae to drugs was evaluated by means of minimal larvicidal concentration (MLC) and larval bursting percentage. MLCs of citral and decanoic acid were almost constant throughout all stages of incubation. However, bursting percentage markedly varied within the first 20 weeks of incubation. Therefore, while larvae are available for use in the MLC assay at any stage of incubation, those beyond the first 20 weeks after incubation should be used for the bursting assay to obtain reproducible results.
犬弓首蛔虫二期幼虫在体外培养了一年。通过最小杀幼虫浓度（MLC）和幼虫破裂率评估幼虫对药物的敏感性。柠檬醛和癸酸的MLC在整个培养阶段几乎保持恒定。然而，在培养的前20周内，破裂率显著变化。因此，虽然在培养的任何阶段幼虫都可用于MLC测定，但培养20周后的幼虫应用于破裂测定以获得可重复的结果。
