在人类免疫缺陷病毒1型（HIV-1）感染者血清中直接检测感染性HIV-1免疫复合物。

Direct detection of infectious human immunodeficiency virus type 1 (HIV-1) immune complexes in the sera of HIV-1-infected persons.

作者信息

Kobayashi K, Takeda A, Green S, Tuazon C U, Ennis F A

机构信息

Dept. of Medicine, University of Massachusetts Medical Center, Worcester 01655.

出版信息

J Infect Dis. 1993 Sep;168(3):729-32. doi: 10.1093/infdis/168.3.729.

DOI:10.1093/infdis/168.3.729

PMID:8354914

Abstract

The sera of human immunodeficiency virus type 1 (HIV-1)-infected subjects were examined for the presence of infectious HIV-1-antibody complexes by their ability to infect Fc gamma receptor (Fc gamma R)-bearing cells. Infection of Fc gamma R-bearing cells by a serum in which half of the p24 antigen was present in a form of immune complexes was inhibited by aggregated human immunoglobulin. Then in studies on 22 sera, 9 sera produced p24 antigen during 14 days of culture in U937 cells. HIV-1 p24 production was inhibited or delayed by the pretreatment of cells with aggregated human immunoglobulin in 6 of the 9 sera that were infectious. These results may reflect interactions between virus-antibody complexes and Fc gamma R-bearing cells in vivo because serum itself was used as the source of virus and virus-antibody complexes. The results indicate that infectious HIV-1 immune complexes are present in the circulation of HIV-1-infected patients.

摘要

通过检测人免疫缺陷病毒1型（HIV-1）感染者血清感染携带Fcγ受体（FcγR）细胞的能力，来检查其中是否存在具有传染性的HIV-1抗体复合物。一种血清中一半的p24抗原以免疫复合物形式存在，该血清对携带FcγR细胞的感染可被人聚合免疫球蛋白抑制。在对22份血清的研究中，9份血清在U937细胞中培养14天期间产生了p24抗原。在9份具有传染性的血清中，有6份血清在用聚合人免疫球蛋白预处理细胞后，HIV-1 p24的产生受到抑制或延迟。由于使用血清本身作为病毒和病毒抗体复合物的来源，这些结果可能反映了病毒抗体复合物与携带FcγR细胞在体内的相互作用。结果表明，具有传染性的HIV-1免疫复合物存在于HIV-1感染患者的循环系统中。