创伤弧菌的荚膜类型：来自临床和环境来源菌株的分析

Capsular types of Vibrio vulnificus: an analysis of strains from clinical and environmental sources.

作者信息

Hayat U, Reddy G P, Bush C A, Johnson J A, Wright A C, Morris J G

机构信息

Department of Medicine, University of Maryland School of Medicine, Baltimore.

出版信息

J Infect Dis. 1993 Sep;168(3):758-62. doi: 10.1093/infdis/168.3.758.

DOI:10.1093/infdis/168.3.758

PMID:8354917

Abstract

Vibrio vulnificus produces a capsular polysaccharide (CPS) that is essential for virulence. CPS from V. vulnificus clinical strain MO6-24 has been purified and the structure determined. In preliminary screening with antisera raised to MO6-24 CPS, 4 (19%) of 21 clinical isolates (including MO6-24), but none of 67 environmental V. vulnificus isolates, agglutinated with anti-MO6-24 antisera (P = .003). CPS was isolated from a subset of 12 clinical and 7 environmental isolates and analyzed by high-performance anion-exchange chromatography and one-dimensional nuclear magnetic resonance. MO6-24 and 1 other serologically positive strain had identical CPS structures; the other 2 serologically positive strains had substitutions in two of four sugar residues. Thirteen other capsular types were identified among the remaining 15 strains from which CPS was extracted.

摘要

创伤弧菌产生一种对毒力至关重要的荚膜多糖（CPS）。已对创伤弧菌临床菌株MO6 - 24的CPS进行了纯化并确定了其结构。在用针对MO6 - 24 CPS产生的抗血清进行的初步筛选中，21株临床分离株（包括MO6 - 24）中有4株（19%）与抗MO6 - 24抗血清发生凝集反应，但67株环境创伤弧菌分离株均未出现这种情况（P = 0.003）。从12株临床分离株和7株环境分离株的一个子集中分离出CPS，并通过高效阴离子交换色谱法和一维核磁共振进行分析。MO6 - 24和另外1株血清学阳性菌株具有相同的CPS结构；其他2株血清学阳性菌株在四个糖残基中的两个上有取代。在从其余15株中提取出CPS的菌株中鉴定出了另外13种荚膜类型。