Kean B H, Kimball A C
Am J Dis Child. 1977 Jan;131(1):21-8. doi: 10.1001/archpedi.1977.02120140023002.
We present serologic results on 26 patients with congenital toxoplasmosis and on 22 of their mothers. The infection was severe (central nervous system involvement) in 12 patients, 12 had only ocular manifestations, and two were asymptomatic. The dye test results were positive on all specimens, and were positive at a titer of 1:1,024 or higher if collected from patients younger than 2 years of age. The complement-fixation test (CFT) results were positive on all specimens from patients younger than 2 years of age and on 69% of specimens collected from older patients. These serologic results are contrasted with those obtained on two control groups: (1) 46 uninfected infants followed up after birth because of substantial antibody titers in their mothers during pregnancy; and (2) 190 infants and children tested because toxoplasmosis was tentatively included in the differential diagnosis of the current illness. In both control groups the positive results on the CFT were limited almost exclusively to cord blood specimens or specimens collected during the first 2 weeks of life. Lower CFT titers in follow-up specimens suggested that the antibodies were maternal in origin. These two tests are valuable in providing laboratory support for the diagnosis of congenital toxoplasmosis, particularly the test for the comparatively short-lived complement-fixing antibody.
我们展示了26例先天性弓形虫病患者及其22位母亲的血清学检测结果。12例患者感染严重（累及中枢神经系统），12例仅有眼部表现，2例无症状。所有标本的染料试验结果均为阳性，若从2岁以下患者采集标本，滴度为1:1024或更高时染料试验结果为阳性。补体结合试验（CFT）结果显示，2岁以下患者的所有标本以及年龄较大患者采集标本的69%呈阳性。这些血清学检测结果与两个对照组的结果形成对比：（1）46例未感染婴儿，因其母亲在孕期抗体滴度较高，出生后进行随访；（2）190例婴儿和儿童，因弓形虫病暂被纳入当前疾病的鉴别诊断而接受检测。在两个对照组中，CFT阳性结果几乎仅局限于脐血标本或出生后前2周采集的标本。随访标本中较低的CFT滴度表明抗体来源于母体。这两项检测对于先天性弓形虫病的诊断提供实验室支持很有价值，尤其是针对相对寿命较短的补体结合抗体的检测。
