Rao R K, Chang H H, Levenson S, Porreca F, Brannon P M, Davis T P, Koldovsky O
Department of Pharmacology, University of Arizona, Tucson.
J Pharmacol Exp Ther. 1993 Aug;266(2):647-54.
Gastric secretions were studied in rats by pyloric ligation in vivo. The effects of epidermal growth factor (EGF) administered s.c. on gastric secretion were evaluated at different stages of development (8, 14, 20 and 30 postnatal days). The rates of fluid, protein and acid outputs were very low in developing rats but increased in maturing rats. The rate of acid output in 8- day-old rats (3.83 +/- 1.16) was not different from that in 14-day-old rats (4.84 +/- 0.99), whereas the rate of acid output in 20-day-old rats was 11-fold greater than that in 14-day-old rats, and it was 21-fold greater in 30-day-old rats. Subcutaneous administration of 30 micrograms/kg b.wt. of EGF significantly suppressed the gastric secretion of fluid, protein and acid in 20- and 30-day-old rats. In 30-day-old rats, acid output was 85% lower at 1 hr after EGF administration and in 20-day-old rats it was 85% lower at 2 hr and 50% lower at 3 hr. Interestingly, EGF had no effect on basal gastric secretions in 8- and 14-day-old rats; EGF also had no effect on the gastric acid output that was stimulated by pentagastrin administration in 14-day-old rats. In 20-day-old rats, inhibitory effects of EGF lasted for 3 hr before gastric secretions rebounded to normal levels, but inhibition lasted for only 1 hr in 30-day-old rats.(ABSTRACT TRUNCATED AT 250 WORDS)
通过在体内进行幽门结扎来研究大鼠的胃分泌情况。在不同发育阶段（出生后8、14、20和30天）评估皮下注射表皮生长因子（EGF）对胃分泌的影响。发育中的大鼠液体、蛋白质和酸的分泌率非常低，但在成熟大鼠中会增加。8日龄大鼠的酸分泌率（3.83±1.16）与14日龄大鼠（4.84±0.99）无差异，而20日龄大鼠的酸分泌率比14日龄大鼠高11倍，30日龄大鼠则高21倍。皮下注射30微克/千克体重的EGF可显著抑制20日龄和30日龄大鼠的胃液、蛋白质和酸分泌。在30日龄大鼠中，注射EGF后1小时酸分泌降低85%，在20日龄大鼠中，2小时降低85%，3小时降低50%。有趣的是，EGF对8日龄和14日龄大鼠的基础胃分泌无影响；EGF对14日龄大鼠注射五肽胃泌素刺激的胃酸分泌也无影响。在20日龄大鼠中，EGF的抑制作用持续3小时，之后胃分泌恢复到正常水平，但在30日龄大鼠中抑制仅持续1小时。（摘要截断于250字）
