链霉菌N174壳聚糖酶的结晶

Crystallization of a chitosanase from Streptomyces N174.

作者信息

Marcotte E, Hart P J, Boucher I, Brzezinski R, Robertus J D

机构信息

Department of Chemistry and Biochemistry, University of Texas, Austin 78712.

出版信息

J Mol Biol. 1993 Aug 5;232(3):995-6. doi: 10.1006/jmbi.1993.1447.

DOI:10.1006/jmbi.1993.1447

PMID:8355284

Abstract

Chitosanases are produced by many soil fungi and bacteria to degrade chitosan present in fungal cell walls. Here, we report the crystallization of a 29,500 dalton protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The crystals were grown by vapor diffusion. They are mechanically strong and diffract to at least 1.9 A resolution. The crystals belong to the monoclinic space group P2(1) with unit cell parameters a = 56.4 A, b = 59.6 A, c = 86.1 A and beta = 96.6 degrees. Cell parameters and crystal density are consistent with two chitosanase molecules per asymmetric unit.

摘要

许多土壤真菌和细菌都会产生壳聚糖酶，用于降解真菌细胞壁中的壳聚糖。在此，我们报告了从链霉菌N174中分离出的一种具有壳聚糖内切水解酶活性、分子量为29,500道尔顿的蛋白质的结晶情况。晶体通过气相扩散法生长。它们机械强度高，衍射分辨率至少可达1.9埃。这些晶体属于单斜空间群P2(1)，晶胞参数为a = 56.4埃，b = 59.6埃，c = 86.1埃，β = 96.6°。晶胞参数和晶体密度与每个不对称单元中有两个壳聚糖酶分子一致。