利用体外化学敏感性试验预测人类白血病的化疗反应。

Prediction of chemotherapy response in human leukemia using in vitro chemosensitivity test.

作者信息

Hwang W S, Chen L M, Huang S H, Wang C C, Tseng M T

机构信息

Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Centre, Taipei, Taiwan.

出版信息

Leuk Res. 1993 Aug;17(8):685-8. doi: 10.1016/0145-2126(93)90074-u.

DOI:10.1016/0145-2126(93)90074-u

PMID:8355513

Abstract

We performed the dye exclusion assay (DEA) and the MTT dye reduction assay to determine the drug sensitivity of acute leukemia using short-term microplate cultures. The in vitro results were compared with the clinical response of 31 patients with acute leukemia treated by combination chemotherapy. The true-positive rates of the DEA and MTT assays were 86.7 and 91.3%, respectively; the true-negative rates were 33.3 and 77.8%, respectively; and the predictive accuracy was 62.5 and 87.5%, respectively. The DEA and MTT assays gave comparable results in drug sensitivity testing of leukemic blast cells. Our data suggest that MTT assay is the more suitable for assessing chemosensitivity in acute leukemia.

摘要

我们使用短期微孔板培养法进行了染料排除试验（DEA）和MTT染料还原试验，以确定急性白血病的药物敏感性。将体外试验结果与31例接受联合化疗的急性白血病患者的临床反应进行比较。DEA试验和MTT试验的真阳性率分别为86.7%和91.3%；真阴性率分别为33.3%和77.8%；预测准确率分别为62.5%和87.5%。在白血病原始细胞的药物敏感性测试中，DEA试验和MTT试验给出了相当的结果。我们的数据表明，MTT试验更适合评估急性白血病的化疗敏感性。