评估MTT法作为急性髓系白血病化疗敏感性快速检测方法的价值。
Appraisal of the MTT assay as a rapid test of chemosensitivity in acute myeloid leukaemia.
作者信息
Sargent J M, Taylor C G
机构信息
Department of Haematology, Pembury Hospital, Kent, UK.
出版信息
Br J Cancer. 1989 Aug;60(2):206-10. doi: 10.1038/bjc.1989.252.
Abstract
We describe the application of a simple, rapid, semi-automated assay to the sensitivity testing of cytotoxic drugs in 23 patients with acute myeloid leukaemia (AML). The survival of blast cells from the bone marrow was measured by the MTT assay after 48 h continuous exposure to drugs both singly and in combination. There was a linear relationship between the number of leukaemic cells and the optical density of the formazan produced. The assay demonstrated a variation in drug sensitivity between patients. The technique was reproducible and there was no significant difference in response between blast cells obtained from bone marrow or from peripheral blood. Preliminary results show a correlation between in vitro and in vivo data. The test can be repeated throughout the course of the disease to help identify any change in tumour sensitivity. This technique appears to give useful information to assist in the management of acute myeloid leukaemia.
摘要
我们描述了一种简单、快速的半自动检测方法在23例急性髓系白血病（AML）患者细胞毒性药物敏感性检测中的应用。将骨髓中的原始细胞连续暴露于单一药物及联合药物48小时后，通过MTT检测法测定其存活率。白血病细胞数量与所产生的甲臜的光密度之间存在线性关系。该检测方法显示出患者之间药物敏感性的差异。该技术具有可重复性，从骨髓或外周血获取的原始细胞在反应上无显著差异。初步结果表明体外和体内数据之间存在相关性。在疾病过程中可重复进行该检测，以帮助识别肿瘤敏感性的任何变化。这项技术似乎能提供有用信息，以协助急性髓系白血病的治疗。
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