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利用酵母中的基因-基因干扰方法在细胞调控网络中实现潜在的快速运作

Potentially rapid walking in cellular regulatory networks using the gene-gene interference method in yeast.

作者信息

Daniel J

机构信息

Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.

出版信息

Mol Gen Genet. 1993 Aug;240(2):245-57. doi: 10.1007/BF00277063.

DOI:10.1007/BF00277063
PMID:8355657
Abstract

The recognition that cellular regulatory circuitry is composed of antagonistic elements has made possible a new approach to the selection of regulatory genes, called the gene-gene interference method. The method was used to identify and isolate genes possibly related in an antagonistic way to protein kinase A. Two such genes were recovered: ART1 encodes a potential regulator of cytokinesis and KAI1 appears to be involved in the Start control. The principles of the gene-gene interference method are discussed, as well as its possible general use for 'walking' within the cellular regulatory networks of eukaryotes.

摘要

认识到细胞调节回路由拮抗元件组成，使得一种新的调节基因选择方法成为可能，这种方法称为基因-基因干扰法。该方法被用于识别和分离可能以拮抗方式与蛋白激酶A相关的基因。回收了两个这样的基因：ART1编码一种胞质分裂的潜在调节因子，而KAI1似乎参与起始控制。本文讨论了基因-基因干扰法的原理，以及它在真核生物细胞调节网络中“游走”的可能普遍用途。

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