De Leonardis A, Casamassima A, Chiuri E, Addabbo L, De Frenza N, Falco G
Unità Operativa di Ginecologia, Istituto Scientifico Oncologico, Bari.
Minerva Ginecol. 1993 Jun;45(6):291-300.
A flow-cytometric analysis of ascitic fluid (AF) and peripheral blood lymphocyte subpopulations (LS) was performed on 16 patients with ovarian malignancy (OM) and 5 with metastatic peritoneal carcinomatosis (MPC). AF lymphocytes are 58% of total leukocytes in OM patients, 37% in MPC patients, while blood lymphocytes are 14% and 17%, respectively. AF absolute lymphocyte count (total and individual LS) is higher in OM patients. OM patients AF lymphocytes are: T = 70%, CD4+ = -37%, CD8+ = 32% (CD4/CD8 = 1.39), B = 5%, NK cells = 4-10%, CD25+ = 20%, CD69+ = 15%, CD71+ = 3%. In MPC patients the values are generally similar, though CD4+ cells are +7%, CD8+ cells = -14% (CD4/CD8 = 3.17), CD69+ cells = -8%. Untreated OM patients have a AF total and percent lymphocyte count higher than treated ones. Among the latter, however, the CD4/CD8 ratio as well as the number of CD4+, inducer, CD25+ and CD71+ cells are higher. In terms of percent values, the most striking differences involve total T and B lymphocytes (81-87% vs 56-62%, and 10% vs 2%, respectively). With the only exception of absolute NK cell count, OM patients show no correlation between AF and peripheral blood LS pattern. These results agree only partially with data from the literature. Pathophysiologic and clinical considerations support the practical usefulness of LS analysis in AF from OM patients.
对16例卵巢恶性肿瘤（OM）患者和5例转移性腹膜癌（MPC）患者的腹水（AF）和外周血淋巴细胞亚群（LS）进行了流式细胞术分析。OM患者腹水中淋巴细胞占白细胞总数的58%，MPC患者为37%，而血液中淋巴细胞分别为14%和17%。OM患者腹水中绝对淋巴细胞计数（总数和各亚群）更高。OM患者腹水中淋巴细胞情况为：T细胞 = 70%，CD4+ = 37%，CD8+ = 32%（CD4/CD8 = 1.39），B细胞 = 5%，NK细胞 = 4 - 10%，CD25+ = 20%，CD69+ = 15%，CD71+ = 3%。MPC患者的数值总体相似，不过CD4+细胞为 +7%，CD8+细胞 = -14%（CD4/CD8 = 3.17），CD69+细胞 = -8%。未经治疗的OM患者腹水中淋巴细胞总数和百分比高于接受治疗的患者。然而，在接受治疗的患者中，CD4/CD8比值以及CD4+、诱导型、CD25+和CD71+细胞的数量更高。就百分比而言，最显著的差异涉及总T淋巴细胞和B淋巴细胞（分别为81 - 87%对56 - 62%，以及10%对2%）。除绝对NK细胞计数外，OM患者腹水中淋巴细胞与外周血淋巴细胞亚群模式之间无相关性。这些结果仅部分与文献数据一致。病理生理和临床考量支持对OM患者腹水中淋巴细胞亚群进行分析具有实际应用价值。
