McAnally J R, Levine M
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Oral Microbiol Immunol. 1993 Apr;8(2):69-74. doi: 10.1111/j.1399-302x.1993.tb00547.x.
Certain monoclonal antibodies (Mabs) neutralize the inhibition of HL60 cell growth by bacterial plaque extracts. The aim of this study was to enumerate and identify the bacteria reactive to these Mabs at sites where periodontal disease was measured by gingival index, pocket depth and attachment loss. The bacterial colonies were grown on blood agar and the colonies counted directly and after immunoblotting. Sites that bled on probing (n = 15) had a mean of 3.35% Mab-positive colonies (SEM = 0.52%) and sites that did not bleed (n = 9) had a mean of 1.61% (SEM = 0.41%). The percentage of Mab-positive colonies also correlated with gingival index but not with the total number of colonies sampled, attachment loss or pocket depth. Antigen was expressed by 4 genera: strains of Streptococcus sanguis and/or Eikenella corrodens (8 sites), Staphylococcus epidermidis (4 sites) and Corynebacterium minutissimus (1 site). The results suggest that the antigens detected on these bacteria by the Mabs may be processed to form toxins and maintain gingivitis by irritating the surrounding cells.
某些单克隆抗体（Mabs）可中和细菌菌斑提取物对HL60细胞生长的抑制作用。本研究的目的是在通过牙龈指数、牙周袋深度和附着丧失来测量牙周疾病的部位，对与这些单克隆抗体发生反应的细菌进行计数和鉴定。细菌菌落生长在血琼脂上，直接计数菌落，并在免疫印迹后计数。探诊出血的部位（n = 15）单克隆抗体阳性菌落的平均比例为3.35%（标准误= 0.52%），未出血的部位（n = 9）平均比例为1.61%（标准误= 0.41%）。单克隆抗体阳性菌落的比例也与牙龈指数相关，但与采样的菌落总数、附着丧失或牙周袋深度无关。有4个菌属表达抗原：血链球菌和/或腐蚀艾肯菌菌株（8个部位）、表皮葡萄球菌（4个部位）和极小棒状杆菌（1个部位）。结果表明，单克隆抗体在这些细菌上检测到的抗原可能被加工形成毒素，并通过刺激周围细胞维持牙龈炎。
