Chen Z F, Shu J, Ma J T, Ni Z M, Mu Z W
Shanghai Institute of Physiology, Chinese Academy of Sciences.
Sheng Li Xue Bao. 1993 Apr;45(2):103-10.
Transpial migration of implanted 5-HT neurons from the subarachnoid space into the spinal cord was studied in adult Wistar rats. Embryonic raphe tissue or cell suspension containing 5-HT cells was used as grafts. The implanted 5-HT cells were monitored by 5-HT immunohistochemical method. The results are as follows: (1) 10 d after cutting the spinal cord at lower thoracic level, 5-HT fibers disappeared in the transected spinal cord. (2) Raphe tissue was implanted into the subarachnoid space of the thoracic lumbar segment after the spinal cord was cut. One month later, 5-HT positive cells could be found in the transected spinal cord with fibers extending into both the grey and the white matters. (3) If the raphe cell suspension instead was implanted, a number of 5-HT positive cells appeared in the grey matter near the implanted region and the distribution of these cells in the grey matter was quite consistent with the implanted range of the cell suspension in the subarachnoid space. The 5-HT positive cells which had entered into the spinal cord sent out fibers and reestablished a new fiber network in the grey matter. (4) After implantation, the number of the 5-HT positive fibers in the transected grey matter became more and more sparsely distributed with increasing distance from the cell bodies and the 5-HT positive fibers reappeared in the white matter were much less than that in the grey matter. Present results show that the implanted 5-HT neurons are able to migrate transpially from the subarachnoid space into the spinal cord.
在成年Wistar大鼠中研究了植入的5-羟色胺（5-HT）神经元从蛛网膜下腔经软膜迁移至脊髓的情况。胚胎中缝组织或含有5-HT细胞的细胞悬液被用作移植物。采用5-HT免疫组织化学方法监测植入的5-HT细胞。结果如下：（1）在胸段下部水平切断脊髓10天后，横断脊髓中的5-HT纤维消失。（2）脊髓切断后，将中缝组织植入胸腰段蛛网膜下腔。1个月后，在横断脊髓中可发现5-HT阳性细胞，其纤维延伸至灰质和白质。（3）若改为植入中缝细胞悬液，则在植入区域附近的灰质中出现大量5-HT阳性细胞，这些细胞在灰质中的分布与细胞悬液在蛛网膜下腔的植入范围相当一致。进入脊髓的5-HT阳性细胞发出纤维，并在灰质中重新建立起新的纤维网络。（4）植入后，横断灰质中5-HT阳性纤维的数量随着与细胞体距离的增加而越来越稀疏地分布，且白质中重新出现的5-HT阳性纤维远少于灰质中的。目前的结果表明，植入的5-HT神经元能够从蛛网膜下腔经软膜迁移至脊髓。
