Harada T, Kim Y, Juhn S K, Sakakura Y
University of Minnesota Otitis Media Research Center, Department of Otolaryngology, University of Minnesota School of Medicine, Minneapolis 55455.
Eur Arch Otorhinolaryngol. 1993;250(3):182-5. doi: 10.1007/BF00171708.
A method for middle ear epithelial (CMEE) cell culture with active mucus secretory function has been successfully developed, using the chinchilla as an animal model. CMEE cells were dissociated by protease digestion from the middle ear mucosa. The CMEE cells grown in primary culture incorporated [3H]glucosamine into a glycoconjugate after its release into medium. This substance was characterized biochemically as mucin, although the production of mucin by the cells required growth on a substratum of collagen gel. These cultures provide an excellent model for studying factors that regulate synthesis and secretion of glycoproteins in CMEE cells.
利用毛丝鼠作为动物模型，已成功开发出一种培养具有活跃黏液分泌功能的中耳上皮（CMEE）细胞的方法。通过蛋白酶消化从中耳黏膜中分离出CMEE细胞。原代培养的CMEE细胞在释放到培养基中后，将[3H]氨基葡萄糖掺入糖缀合物中。尽管细胞产生黏蛋白需要在胶原凝胶基质上生长，但这种物质经生化鉴定为黏蛋白。这些培养物为研究调节CMEE细胞中糖蛋白合成和分泌的因素提供了一个极好的模型。
