细胞因子、抗癌剂和辅助致癌物对毛球细胞DNA合成的影响。

Effects of cytokines, anti-cancer agents and cocarcinogen on DNA synthesis in hair bulb cells.

作者信息

Imai R, Jindo T, Mochida K, Shimaoka S, Takamori K, Ogawa H

机构信息

Department of Dermatology, Juntendo University School of Medicine, Tokyo, Japan.

出版信息

J Dermatol Sci. 1993 Apr;5(2):73-80. doi: 10.1016/0923-1811(93)90073-x.

DOI:10.1016/0923-1811(93)90073-x

PMID:8357784

Abstract

We analysed the effects of cytokines, anti-cancer agents and cocarcinogen on DNA synthesis in human hair germinative cells cultured in serum-free media. Epidermal growth factor and gamma interferon were found to inhibit DNA synthesis slightly, while strong inhibition was demonstrated by doxorubicin, cytosine arabinoside and tetradecanoyl-phorbolacetate. Basic fibroblast growth factor had very little influence on DNA synthesis. This organ culture model in serum-free media is a useful method by which to examine the effects of various cytokines and drugs on DNA synthesis in hair germinative cells and/or to study the pathogenesis of various alopecia diseases.

摘要

我们分析了细胞因子、抗癌药物和促癌物对在无血清培养基中培养的人毛发生殖细胞DNA合成的影响。发现表皮生长因子和γ干扰素对DNA合成有轻微抑制作用，而阿霉素、阿糖胞苷和十四酰佛波醇乙酸酯则表现出强烈抑制作用。碱性成纤维细胞生长因子对DNA合成影响很小。这种无血清培养基中的器官培养模型是一种有用的方法，可用于研究各种细胞因子和药物对毛发生殖细胞DNA合成的影响，以及研究各种脱发疾病的发病机制。