Sakakura C, Takahashi T, Hagiwara A, Yamane T, Sawai S, Okano S, Ozaki K, Sasaki S, Tsujimoto H
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Anticancer Drugs. 1993 Jun;4(3):381-8. doi: 10.1097/00001813-199306000-00016.
A single dose of inactivated streptococci (OK-432) was injected into the popliteal lymph nodes of male CDF1 mice and its effects on popliteal, inguinal, and para-aortic lymph node cells and spleen cells were investigated and compared with the effects of subcutaneous injections of the same dosage of OK-432. Regional lymph node cells and spleen cells obtained from intralymphnodally injected mice lysed not only natural killer (NK)-sensitive YAC-1 cells, but also NK-resistant P-815 and meth-A cells. Lysis of target cells was inhibited when effector cells were treated with anti-Thy-1.2 or anti-Lyt-2.2 monoclonal antibody and complement, but no inhibition was apparent after treatment with anti-asialo-GM1 or anti-Lyt-1.2 antibody and complement. These results suggest that the effector cells are lymphocyte-activated killer (LAK) cells. An enhanced capacity of lymph node cells to produce cytokines, tumor necrosis factor and interleukin 1 upon restimulation with lipopolysaccharide was found only in intralymphnodally injected mice. Thus, the induction of LAK-like cells and cytokine production in regional lymph nodes and spleen cells by the intralymphnodal administration of OK-432 should be effective for the inhibition or treatment of lymph node metastases.
将单剂量的灭活链球菌（OK-432）注射到雄性CDF1小鼠的腘窝淋巴结中，研究其对腘窝、腹股沟及主动脉旁淋巴结细胞和脾细胞的影响，并与皮下注射相同剂量OK-432的效果进行比较。从经淋巴结内注射的小鼠获得的局部淋巴结细胞和脾细胞不仅能裂解自然杀伤（NK）敏感的YAC-1细胞，还能裂解NK抗性的P-815细胞和甲基胆蒽诱导的肉瘤（meth-A）细胞。当用抗Thy-1.2或抗Lyt-2.2单克隆抗体及补体处理效应细胞时，靶细胞的裂解受到抑制，但用抗去唾液酸GM1或抗Lyt-1.2抗体及补体处理后未见明显抑制。这些结果表明效应细胞是淋巴细胞激活的杀伤（LAK）细胞。仅在经淋巴结内注射的小鼠中发现，用脂多糖再次刺激时，淋巴结细胞产生细胞因子、肿瘤坏死因子和白细胞介素1的能力增强。因此，通过淋巴结内给予OK-432诱导局部淋巴结和脾细胞中LAK样细胞和细胞因子产生，对抑制或治疗淋巴结转移应是有效的。
