Bolender R P, Charleston J S
Department of Biological Structure, University of Washington School of Medicine, Seattle 98195.
Microsc Res Tech. 1993 Jul 1;25(4):314-24. doi: 10.1002/jemt.1070250408.
We describe MS-DOS software for the optical volume fractionator (OVF), a stereological method combining the principles of the optical disector (Gundersen et al.: Acta Pathol. Microbiol. Immunol. Scand., 96:857-881, 1988) and fractionator (Gundersen: J. Microsc., 143:3-45, 1986). The OVF program estimates the volume of a fixed and embedded structure, the numerical density of cells, and the total number of cells in a structure. The hardware requirements include a PC computer (386 or 486 with VGA graphics) and a conventional light microscope fitted with a rotating stage, extension tube, and length gauge. The software includes an introduction, tutorial, simulator, laboratory tool kit, and report generator. The tool kit improves the efficiency of gathering stereological data with light microscopy and offers a convenient link between the data of light and electron microscopy. A novel algorithm, based on fractionator sampling, gives the volume of a fixed and embedded structure from the same set of sections used for cell counting. A laboratory example illustrates the operation of the software.
我们描述了用于光学体积分数法（OVF）的MS-DOS软件，光学体积分数法是一种将光学分割法（贡德森等人：《病理学、微生物学与免疫学文献，斯堪的纳维亚分册》，96:857 - 881，1988年）和分数法（贡德森：《显微镜学杂志》，143:3 - 45，1986年）原理相结合的体视学方法。OVF程序可估计固定和包埋结构的体积、细胞的数字密度以及结构中细胞的总数。硬件要求包括一台个人计算机（386或486且具备VGA图形）以及一台配备旋转载物台、延长管和长度测量仪的传统光学显微镜。该软件包括引言、教程、模拟器、实验室工具包和报告生成器。工具包提高了通过光学显微镜收集体视学数据的效率，并在光学和电子显微镜数据之间提供了便捷的链接。一种基于分数法采样的新颖算法可从用于细胞计数的同一组切片中得出固定和包埋结构的体积。一个实验室实例说明了该软件的操作。
