Wells U M, Yurdakos E, Kitano S, Webber S E, Widdicombe J G
Department of Physiology, St George's Hospital Medical School, Tooting, London, UK.
Pulm Pharmacol. 1993 Jun;6(2):137-42. doi: 10.1006/pulp.1993.1017.
In the ferret liquid-filled trachea in vivo, intraluminal bradykinin (BK, 3-300 microM) produced concentration-dependent increases in the output of lysozyme from submucosal gland serous cells and albumin movement into the lumen. Baseline outputs of albumin and lysozyme were not altered significantly by intraluminal indomethacin (10 microM) or thiorphan (10 microM). However, intraluminal indomethacin completely blocked the BK-induced increase in albumin output. Intraluminal thiorphan (10 microM) did not significantly potentiate BK-induced albumin output, although mean output was higher. Neither indomethacin nor thiorphan significantly altered BK-induced lysozyme output, although mean output was reduced in the presence of indomethacin. Thus BK increases albumin output and may increase lysozyme output via the action of cyclooxygenase products. Inhibition of neutral endopeptidase activity may enhance the action of BK on albumin output.
在雪貂体内充满液体的气管中，管腔内缓激肽（BK，3 - 300微摩尔）使来自黏膜下腺浆液细胞的溶菌酶分泌量和白蛋白向管腔内的移动呈浓度依赖性增加。管腔内吲哚美辛（10微摩尔）或硫氧还蛋白（10微摩尔）对白蛋白和溶菌酶的基础分泌量无显著影响。然而，管腔内吲哚美辛完全阻断了BK诱导的白蛋白分泌增加。管腔内硫氧还蛋白（10微摩尔）虽使平均分泌量较高，但并未显著增强BK诱导的白蛋白分泌。吲哚美辛和硫氧还蛋白均未显著改变BK诱导的溶菌酶分泌，不过在吲哚美辛存在时平均分泌量有所降低。因此，BK可增加白蛋白分泌，且可能通过环氧化酶产物的作用增加溶菌酶分泌。抑制中性内肽酶活性可能增强BK对白蛋白分泌量的作用。
