Hartmann H J, Felix K, Nagel W, Weser U
Anorganische Biochemie, Physiologisch-Chemisches Institut, Eberhard-Karls-Universität Tübingen, Germany.
Biometals. 1993 Summer;6(2):115-8. doi: 10.1007/BF00140112.
The molecular side of copper transport in biological systems is unknown. It was attempted to examine the copper and metallothionein (MT) release into the portal blood in rats in vivo. After direct administration of Cu(II) into the jejunum the copper and MT levels were distinctively higher in the portal venous serum compared with that of the vena cava inferior. MT in gel filtrated serum samples was analyzed immunologically employing ELISA and a monoclonal antibody to rat MT-I. Affinity chromatography on Protein A-Sepharose resulted in a higher immunoreactivity in the portal compartment as deduced from an elevated MT-antibody complex. It is assumed that MT serves as a genuine transport system for cuprous copper during the mucosal-to-serosal flux of this biologically important transition metal.
生物系统中铜转运的分子机制尚不清楚。本研究试图检测大鼠体内门静脉血中铜和金属硫蛋白（MT）的释放情况。将Cu(II)直接注入空肠后，门静脉血清中的铜和MT水平明显高于下腔静脉血清。采用酶联免疫吸附测定（ELISA）和抗大鼠MT-I单克隆抗体对凝胶过滤血清样本中的MT进行免疫分析。通过蛋白A-琼脂糖亲和层析，MT-抗体复合物水平升高，表明门静脉部分具有更高的免疫反应性。推测在这种生物重要过渡金属从黏膜到浆膜的转运过程中，MT作为亚铜的真正转运系统发挥作用。
