Antoniazzi F, Radetti G, Zamboni G, Gambaro G, Adami S, Tatò L
Servizio Autonomo di Endocrinologia Pediatrica, University of Verona, Italy.
Bone Miner. 1993 May;21(2):151-6. doi: 10.1016/s0169-6009(08)80017-7.
In order to investigate the influence on bone metabolism of growth hormone (GH), we evaluated the response to an acute load of 1,25(OH)2D3 (Rocaltrol) (1.5 micrograms/day for 4 days) in 16 growth hormone-deficient prepubertal children (11 boys and 5 girls, aged from 6.2 to 9.6 years) both before and after 1 month of human GH (hGH) therapy (0.1 IU/kg/day, 6 times per week). Before and after the 1,25(OH)2D3 load, serum IGF-I, osteocalcin, Ca, P, alkaline phosphatase (ALP) and urinary excretion of Ca and P were measured. The serum levels of osteocalcin rose significantly after the first 1,25(OH)2D3 load, without an increase in IGF-I values and with a slight non-significant increase in serum Ca and P. Almost superimposable increases of osteocalcin, Ca and P were observed after 1 month of hGH therapy, with a significant increase of IGF-I, but they did not rise further after the second 1,25(OH)2D3 load. On the basis of our results, 1,25(OH)2D3 seems to have a stimulatory action on osteoblastic activity even in the absence of normal levels of GH. However, there is no apparent additional stimulatory activity after administration of hGH. Osteocalcin level behaviour during our study might suggest that GH and 1,25(OH)2D3 have a common and easily saturable stimulatory pathway on osteoblastic function.
为了研究生长激素（GH）对骨代谢的影响，我们评估了16名青春期前生长激素缺乏儿童（11名男孩和5名女孩，年龄6.2至9.6岁）在接受1个月的人生长激素（hGH）治疗（0.1 IU/kg/天，每周6次）前后，对1,25(OH)2D3（罗钙全）急性负荷（1.5微克/天，共4天）的反应。在给予1,25(OH)2D3负荷前后，测量血清胰岛素样生长因子-I（IGF-I）、骨钙素、钙、磷、碱性磷酸酶（ALP）以及钙和磷的尿排泄量。首次给予1,25(OH)2D3负荷后，血清骨钙素水平显著升高，IGF-I值未增加，血清钙和磷略有非显著性升高。hGH治疗1个月后，观察到骨钙素、钙和磷几乎有叠加性升高，IGF-I显著增加，但第二次给予1,25(OH)2D3负荷后它们未进一步升高。根据我们的结果，即使在GH水平不正常的情况下，1,25(OH)2D3似乎对成骨细胞活性也有刺激作用。然而，给予hGH后没有明显的额外刺激活性。我们研究期间骨钙素水平的变化可能表明，GH和1,25(OH)2D3在成骨细胞功能上有共同且易于饱和的刺激途径。
