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Microheterogeneity in O-type sugar chains of carbohydrases secreted by Asp. awamori.

作者信息

Neustroev K N, Golubev A M, Ibatullin F M, Moseichuk A V

机构信息

Dept. of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russia.

出版信息

Biochem Mol Biol Int. 1993 May;30(1):107-13.

PMID:8358322
Abstract

This paper deals with microheterogeneity in the structure of O-linked sugars of carbohydrases secreted by Asp. awamori, namely glucoamylase, alpha-galactosidase and alpha-glucosidase. Microheterogeneity was found to be related both to post-secretion deglycosylation and to changes in transferase activity induced by the differences in culturing conditions.

摘要

相似文献

1
Microheterogeneity in O-type sugar chains of carbohydrases secreted by Asp. awamori.
Biochem Mol Biol Int. 1993 May;30(1):107-13.
2
Refined crystal structures of glucoamylase from Aspergillus awamori var. X100.泡盛曲霉变种X100葡萄糖淀粉酶的精细晶体结构
J Mol Biol. 1994 May 13;238(4):575-91. doi: 10.1006/jmbi.1994.1316.
3
Effect of modification of carbohydrate component on properties of glucoamylase.
FEBS Lett. 1993 Jan 25;316(2):157-60. doi: 10.1016/0014-5793(93)81206-f.
4
[Carboxyl groups in the active center of glucoamylase from Aspergillus awamori].[泡盛曲霉糖化酶活性中心的羧基]
Biokhimiia. 1982 Oct;47(10):1618-20.
5
[Effect of modifications of a series of amino acid radicals on the enzymatic activity of glucoamylase from Aspergillus awamori].[一系列氨基酸基团修饰对泡盛曲霉葡糖淀粉酶酶活性的影响]
Biokhimiia. 1983 Aug;48(8):1311-8.
6
Adsorption of glucoamylase from Asp. awamori X-100/D27 on cell walls.泡盛曲霉X-100/D27的糖化酶在细胞壁上的吸附作用。
Biochem Mol Biol Int. 1993 May;30(1):115-20.
7
Expression in Pichia pastoris and purification of Aspergillus awamori glucoamylase catalytic domain.泡盛曲霉糖化酶催化结构域在毕赤酵母中的表达及纯化
Protein Expr Purif. 1997 Jun;10(1):70-9. doi: 10.1006/prep.1996.0713.
8
Functional and structural roles of the highly conserved Trp120 loop region of glucoamylase from Aspergillus awamori.泡盛曲霉葡糖淀粉酶高度保守的Trp120环区域的功能和结构作用
Biochemistry. 1996 Mar 5;35(9):3050-8. doi: 10.1021/bi952458x.
9
[Carbohydrates of the medium and the nature of ontogeny in Aspergillus awamori in relation to glucoamylase biosynthesis].[泡盛曲霉中培养基碳水化合物与个体发育性质及糖化酶生物合成的关系]
Mikrobiologiia. 1980 Jul-Aug;49(4):527-33.
10
Overexpression and characterization of Aspergillus awamori wild-type and mutant glucoamylase secreted by the methylotrophic yeast Pichia pastoris: comparison with wild-type recombinant glucoamylase produced using Saccharomyces cerevisiae and Aspergillus niger as hosts.甲基营养型酵母毕赤酵母分泌的泡盛曲霉野生型和突变型糖化酶的过表达及特性分析:与以酿酒酵母和黑曲霉为宿主生产的野生型重组糖化酶的比较
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