Carriere F, Barrowman J A, Verger R, Laugier R
Laboratoire de Lipolyse Enzymatique, Centre National de la Recherche Scientifique, Marseille, France.
Gastroenterology. 1993 Sep;105(3):876-88. doi: 10.1016/0016-5085(93)90908-u.
The aim of this study was to quantitatively evaluate the relative contributions to in vivo lipolysis of gastric and pancreatic lipases.
Gastric and pancreatic lipase secretions were measured, and their respective levels were determined in duodenal fluid during the digestion of a liquid test meal in healthy volunteers. Gastric lipase activity was clearly distinguished from that of pancreatic lipase by using both a specific enzymatic assay and an enzyme-linked immunosorbent assay. Lipolysis products were monitored throughout the digestion period.
On a weight basis, the ratio of pancreatic lipase to gastric lipase total secretory outputs was found to be around four after 3 hours of digestion. The level of gastric hydrolysis was calculated to be 10% +/- 1% of the acyl chains released from the meal triglycerides. Gastric lipase remained active in the duodenum where it might still hydrolyze 7.5% of the triglyceride acyl chains.
Globally during the whole digestion period, gastric lipase might hydrolyze 17.5% of the triglyceride acyl chains. In other words, gastric lipase might hydrolyze 1 acyl chain of 4, which need to be hydrolyzed for a complete intestinal absorption of monoglycerides and free fatty acids resulting from the degradation of two triglyceride molecules.
本研究的目的是定量评估胃脂肪酶和胰脂肪酶对体内脂肪分解的相对贡献。
测量了健康志愿者在消化液体试验餐期间十二指肠液中胃脂肪酶和胰脂肪酶的分泌量，并确定了它们各自的水平。通过使用特异性酶测定法和酶联免疫吸附测定法，将胃脂肪酶活性与胰脂肪酶活性清楚地区分开来。在整个消化期间监测脂肪分解产物。
以重量计，消化3小时后，胰脂肪酶与胃脂肪酶总分泌量的比率约为4。计算得出胃水解水平占膳食甘油三酯释放的酰基链的10%±1%。胃脂肪酶在十二指肠中仍保持活性，在那里它仍可能水解7.5%的甘油三酯酰基链。
在整个消化期间，总体而言，胃脂肪酶可能水解17.5%的甘油三酯酰基链。换句话说，胃脂肪酶可能水解4个酰基链中的1个，而这是两个甘油三酯分子降解产生的甘油单酯和游离脂肪酸完全被肠道吸收所需要水解的。
