Rockabrand D, Partridge J, Krska J, Blum P
School of Biological Science, University of Nebraska, Lincoln 68588-0118.
FEMS Microbiol Lett. 1993 Jul 15;111(1):79-85. doi: 10.1111/j.1574-6968.1993.tb06365.x.
DNA sequence analysis of chromosomal DNA from the Gram-positive facultative intracellular pathogen, Erysipelothrix rhusiopathiae has identified a dnaJ heat shock gene homolog. A 1109-bp open reading frame encoding dnaJ is located immediately 3' to the E. rhusiopathiae dnaK gene. The deduced DnaJ amino acid sequence exhibits the modular structure of other members of the DnaJ protein class including a glycine-rich region and the repeating consensus sequence CXXCXGXGX. Heterologous expression of the dnaJ sequence in Escherichia coli resulted in accumulation of a unique 38.9-kDa protein with an isoelectric point of 8.0. Deletion analysis of the dnaJ gene was used to confirm that the overproduced protein was encoded by the dnaJ sequence.
对革兰氏阳性兼性胞内病原体猪红斑丹毒丝菌的染色体DNA进行的DNA序列分析，鉴定出一个dnaJ热休克基因同源物。一个编码dnaJ的1109 bp开放阅读框紧邻猪红斑丹毒丝菌dnaK基因的3'端。推导的DnaJ氨基酸序列具有DnaJ蛋白家族其他成员的模块化结构，包括一个富含甘氨酸的区域和重复的共有序列CXXCXGXGX。dnaJ序列在大肠杆菌中的异源表达导致积累了一种独特的38.9 kDa蛋白质，其等电点为8.0。对dnaJ基因进行缺失分析以确认过量产生的蛋白质是由dnaJ序列编码的。
