Schindler M, Mach R L, Vollenhofer S K, Hodits R, Gruber F, Visser J, De Graaff L, Kubicek C P
Abteilung für Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Vienna, Austria.
Gene. 1993 Aug 25;130(2):271-5. doi: 10.1016/0378-1119(93)90430-b.
The pyruvate kinase-encoding gene (pki1) from Trichoderma reesei was isolated by hybridization to the corresponding Aspergillus nidulans pkiA gene. The 1614-bp nucleotide (nt) sequence of the cloned gene codes for a 538-amino-acid protein. The coding sequence contains a single intron of 246 nt at a position identical to that of intron E in the A. nidulans gene. The PKI protein shows extensive homology to the PKIs of A. nidulans and A. niger (67%) and Saccharomyces cerevisiae (59%). The 5' non-coding sequence contains a number of motifs typical for yeast glycolytic genes, but so far only rarely found in filamentous fungi.
通过与构巢曲霉的相应pkiA基因杂交，分离得到了里氏木霉的丙酮酸激酶编码基因（pki1）。克隆基因的1614个碱基对（nt）序列编码一个538个氨基酸的蛋白质。编码序列在与构巢曲霉基因中内含子E相同的位置含有一个246 nt的单一内含子。PKI蛋白与构巢曲霉和黑曲霉的PKI（67%）以及酿酒酵母的PKI（59%）具有广泛的同源性。5'非编码序列包含许多酵母糖酵解基因典型的基序，但迄今为止在丝状真菌中很少发现。
