Fujisawa R, Butler W T, Brunn J C, Zhou H Y, Kuboki Y
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
J Dent Res. 1993 Aug;72(8):1222-6. doi: 10.1177/00220345930720081001.
Matrices of dentin and bone were compared with respect to the content of cell-attachment sialoproteins. The levels of two sialoproteins, osteopontin (OPN) and bone sialoprotein (BSP), were determined in dentin and bone by immunochemical procedures. Polyclonal antibodies against bovine BSP and an antibody against the amino-terminal decapeptide of rat OPN were used. The relative levels of OPN and BSP in dentin were less than one-tenth of the levels in bone. The differences between dentin and bone levels of OPN and BSP were thus larger than those for osteonectin or bone Gla protein in the two tissues. The scarcity of the cell-attachment proteins in dentin may reflect the metabolic inactivity of dentin.
就细胞黏附唾液蛋白的含量对牙本质和骨的基质进行了比较。通过免疫化学方法测定了牙本质和骨中两种唾液蛋白——骨桥蛋白（OPN）和骨唾液蛋白（BSP）的水平。使用了针对牛BSP的多克隆抗体和针对大鼠OPN氨基末端十肽的抗体。牙本质中OPN和BSP的相对水平不到骨中水平的十分之一。因此，牙本质和骨中OPN和BSP水平的差异大于这两种组织中骨连接素或骨钙蛋白的差异。牙本质中细胞黏附蛋白的缺乏可能反映了牙本质的代谢不活跃。
