Crispino M, Capano C P, Kaplan B B, Giuditta A
Dipartimento di Fisiologia Generale e Ambientale, Università di Napoli, Italy.
J Neurochem. 1993 Sep;61(3):1144-6. doi: 10.1111/j.1471-4159.1993.tb03632.x.
It is generally believed that the proteins of the nerve endings are synthesized on perikaryal polysomes and are eventually delivered to the presynaptic domain by axoplasmic flow. At variance with this view, we have reported previously that a synaptosomal fraction from squid brain actively synthesizes proteins whose electrophoretic profile differs substantially from that of the proteins made in nerve cell bodies, axons, or glial cells, i.e., by the possible contaminants of the synaptosomal fraction. Using western analyses and immunoabsorption methods, we report now that (a) the translation products of the squid synaptosomal fraction include neurofilament (NF) proteins and (b) the electrophoretic pattern of the synaptosomal newly synthesized NF proteins is drastically different from that of the NF proteins synthesized by nerve cell bodies. The latter results exclude the possibility that NF proteins synthesized by the synaptosomal fraction originate in fragments of nerve cell bodies possibly contaminating the synaptosomal fraction. They rather indicate that in squid brain, nerve terminals synthesize NF proteins.
人们普遍认为，神经末梢的蛋白质是在胞体多核糖体上合成的，最终通过轴浆运输被运送到突触前区域。与这种观点不同的是，我们之前报道过，来自鱿鱼大脑的突触体组分能够活跃地合成蛋白质，其电泳图谱与神经细胞体、轴突或胶质细胞（即突触体组分可能的污染物）中合成的蛋白质有很大差异。利用蛋白质免疫印迹分析和免疫吸附方法，我们现在报告：（a）鱿鱼突触体组分的翻译产物包括神经丝（NF）蛋白；（b）突触体新合成的NF蛋白的电泳图谱与神经细胞体合成的NF蛋白有很大不同。后一个结果排除了突触体组分合成的NF蛋白源自可能污染突触体组分的神经细胞体片段的可能性。它们反而表明，在鱿鱼大脑中，神经末梢能够合成NF蛋白。
