Passaquin A C, Metzinger L, Léger J J, Warter J M, Poindron P
Département d'Immunologie, Immunopharmacologie et Pathologie, Université Louis Pasteur, Illkirch, France.
J Neurosci Res. 1993 Jul 1;35(4):363-72. doi: 10.1002/jnr.490350403.
The differentiation of skeletal muscle cells from mdx mice which lack dystrophin expression was examined after glucocorticoid treatment, namely alpha-methylprednisolone (PDN). Primary skeletal muscle cell cultures were established from newborn mdx, congenic C57BL/10, and allogenic BALB/C mice. We show that PDN promotes the myogenesis of both mdx- and control mice-derived cultures as determined by 1) the number of myotubes, 2) acetylcholine receptors, and 3) dystrophin and dystrophin-related protein levels. These results support the hypothesis that PDN could enhance the myogenesis of satellite cells and increase dystrophin-related protein expression in DMD treated patients.
在给予糖皮质激素治疗（即α-甲基泼尼松龙，PDN）后，研究了缺乏抗肌萎缩蛋白表达的mdx小鼠骨骼肌细胞的分化情况。从新生mdx小鼠、同基因C57BL/10小鼠和同种异体BALB/C小鼠建立了原代骨骼肌细胞培养物。我们发现，通过以下三个指标确定，PDN可促进mdx小鼠和对照小鼠来源培养物的肌生成：1）肌管数量；2）乙酰胆碱受体；3）抗肌萎缩蛋白和抗肌萎缩蛋白相关蛋白水平。这些结果支持这样的假说，即PDN可增强卫星细胞的肌生成，并增加杜氏肌营养不良症（DMD）患者中抗肌萎缩蛋白相关蛋白的表达。
