Chimura T, Hirayama T, Nakahara M
Department of Obstetrics and Gynecology, School of Medicine, Yamagata University.
Jpn J Antibiot. 1993 Jun;46(6):482-5.
As one of the biodefense mechanisms, lactoferrin (LFN) in the secreta of female genital organ may be an interesting biological material in view of its antimicrobial activity. In the present study, we investigated antimicrobial activities of LFN and its combination with cefpodoxime proxetil (CPDX-PR), we also as evaluated clinical effect of CPDX-PR. The following results were obtained. 1. Antimicrobial activities of LFN were tested against 15 strains of 10 species of bacteria, and potent activities against Staphylococcus aureus 209P, Escherichia coli, Klebsiella pneumoniae and Proteus spp. were found. 2. In a concomitant use of LFN with CPDX-PR (a checkerboard method), synergistic actions were observed against S. aureus 209P, E. coli STf, K. pneumoniae 602 and Pseudomonas aeruginosa 1046, and additive actions against E. coli NIHJ and Providencia rettgeri 1603. In 3 strains, the MICs of CPDX-PR in the presence of LFN were reduced to < 1/64. 3. In the evaluation of clinical effect of CPDX-PR, efficacy rates were 53/57 (92.9%) in a patient group with infections. The incidence of adverse reaction was 0/57.
作为生物防御机制之一，女性生殖器官分泌物中的乳铁蛋白（LFN）因其抗菌活性可能是一种有趣的生物材料。在本研究中，我们研究了LFN及其与头孢泊肟酯（CPDX-PR）联合使用的抗菌活性，还评估了CPDX-PR的临床效果。获得了以下结果。1. 测试了LFN对10种细菌的15个菌株的抗菌活性，发现其对金黄色葡萄球菌209P、大肠杆菌、肺炎克雷伯菌和变形杆菌属有较强活性。2. 在LFN与CPDX-PR联合使用时（棋盘法），观察到对金黄色葡萄球菌209P、大肠杆菌STf、肺炎克雷伯菌602和铜绿假单胞菌1046有协同作用，对大肠杆菌NIHJ和雷氏普罗威登斯菌1603有相加作用。在3个菌株中，LFN存在时CPDX-PR的最低抑菌浓度降至<1/64。3. 在评估CPDX-PR的临床效果时，感染患者组的有效率为53/57（92.9%）。不良反应发生率为0/57。
