Yano H, Takenaka H, Onitsuka T, Koga Y, Hamada M
Second Department of Surgery, Miyazaki Medical College, Japan.
J Thorac Cardiovasc Surg. 1993 Sep;106(3):502-10.
The effectiveness of the University of Wisconsin solution and the Collins' M solution for preservation of rat hearts was compared by examining histologic appearance, tissue water content, and mitochondrial respiratory functions after prolonged hypothermic storage and subsequent heterotopic transplantation. Survival of transplanted hearts after 5 days of reperfusion was markedly lowered by storage in Collins' M solution for 15 hours. Hearts stored in University of Wisconsin solution for 10 hours showed no increase in myocardial necrosis after 5 days of reperfusion, whereas hearts stored in University of Wisconsin solution for 15 hours and Collins' M solution for 10 and 15 hours showed a significant increase in tissue necrosis. University of Wisconsin solution reduced tissue swelling during hypothermic storage, whereas Collins' M solution did not cause such reduction. The yield of mitochondrial protein after reperfusion was significantly decreased by storage in either solution, especially after 15 hours in Collins' M solution. Mitochondrial oxidative phosphorylation was significantly inhibited by storage, especially by storage in Collins' M solution and subsequent reperfusion. These results indicate that myocardial injury, after prolonged ischemia and reperfusion, results in a decrease in functionally and structurally intact mitochondria that is dependent on preservation conditions. University of Wisconsin solution protects isolated hearts against ischemia and reperfusion injury possibly by preventing cellular and mitochondrial deterioration.
通过在长时间低温保存及随后的异位移植后检查组织学外观、组织含水量和线粒体呼吸功能，比较了威斯康星大学溶液和柯林斯M溶液对大鼠心脏的保存效果。在柯林斯M溶液中保存15小时后，移植心脏再灌注5天后的存活率显著降低。在威斯康星大学溶液中保存10小时的心脏在再灌注5天后心肌坏死没有增加，而在威斯康星大学溶液中保存15小时以及在柯林斯M溶液中保存10小时和15小时的心脏组织坏死显著增加。威斯康星大学溶液在低温保存期间减少了组织肿胀，而柯林斯M溶液没有导致这种减少。再灌注后线粒体蛋白产量在两种溶液中保存后均显著降低，尤其是在柯林斯M溶液中保存15小时后。线粒体氧化磷酸化在保存后显著受到抑制，尤其是在柯林斯M溶液中保存并随后再灌注后。这些结果表明，长时间缺血和再灌注后的心肌损伤导致功能和结构完整的线粒体减少，这取决于保存条件。威斯康星大学溶液可能通过防止细胞和线粒体退化来保护离体心脏免受缺血和再灌注损伤。
