Sangameswaran L, Morgan J I
Department of Neuroscience, Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
Brain Res Mol Brain Res. 1993 Jul;19(1-2):62-8. doi: 10.1016/0169-328x(93)90149-j.
PEP-19 is a 61 amino acid polypeptide that is localized to neurons. PEP-19 is translated from a 0.6 kb poly(A)+ RNA; however, the gene from which it is transcribed spans more than 30 kbp and comprises three exons and two large introns. Exon 1 contains the 5'-untranslated region of PEP-19 and the first three amino acids of the coding sequence; exon 2 codes for the next 17 amino acids while the majority of the PEP-19 mRNA, comprising the remaining 42 amino acids and the 3'-untranslated region, is in exon 3. Gel retardation analysis identifies a region of DNA in the putative promoter of PEP-19 that binds preferentially to nuclear extracts from cerebellum. However, constructs containing 1.35 kbp of 5'-upstream genomic DNA of PEP-19 fused to lacZ do not express in transgenic mice, suggesting that intragenic DNA may be essential for the regulation of PEP-19.
PEP - 19是一种定位于神经元的61个氨基酸的多肽。PEP - 19由0.6 kb的多聚腺苷酸加尾RNA翻译而来；然而，其转录基因跨度超过30 kbp，包含三个外显子和两个大的内含子。外显子1包含PEP - 19的5'非翻译区和编码序列的前三个氨基酸；外显子2编码接下来的17个氨基酸，而包含其余42个氨基酸和3'非翻译区的大部分PEP - 19 mRNA位于外显子3中。凝胶阻滞分析确定了PEP - 19假定启动子中的一个DNA区域，该区域优先与小脑的核提取物结合。然而，含有与lacZ融合的PEP - 19的1.35 kbp 5'上游基因组DNA的构建体在转基因小鼠中不表达，这表明基因内DNA可能对PEP - 19的调控至关重要。
