Ravinsky E, Quinonez G E, Paraskevas M, Diocee M S, Kutryk E M
Department of Pathology, University of Manitoba Health Sciences Centre, Winnipeg, Canada.
Acta Cytol. 1993 Sep-Oct;37(5):661-6.
While processing needle rinses from fine needle aspiration biopsy material for electron microscopy, we experienced difficulties not fully outlined previously. Problems in preservation were traced to the time elapsed before glutaraldehyde was added to the needle rinse, which had been held in a physiologic transport medium; a tendency toward hypotonicity of the commercially prepared transport medium; and vigorous handling of the needle rinse. When these problems were dealt with, preservation improved dramatically. The problem of scanty material available for ultrastructural evaluation was dealt with by decreasing the number of smears made and filtering the needle rinse to capture tissue fragments. Implementation of the procedure required leadership, good communication, motivated cytotechnologists and cytopathologists, and continuous supervision and monitoring. In cases with adequately cellular well-preserved material available for electron microscopy, ultrastructural evaluation made a contribution of clinical value to the diagnosis in 48% of cases.
在处理用于电子显微镜检查的细针穿刺活检材料的针冲洗液时，我们遇到了一些以前未完全阐述清楚的困难。保存方面的问题可追溯到在将针冲洗液置于生理转运介质中后，直至添加戊二醛之前所经过的时间；市售制备的转运介质有出现低渗的倾向；以及对针冲洗液的大力处理。当这些问题得到解决后，保存情况有了显著改善。对于超微结构评估可用材料稀少的问题，通过减少涂片数量并过滤针冲洗液以捕获组织碎片来解决。该程序的实施需要领导能力、良好的沟通、积极主动的细胞技术人员和细胞病理学家，以及持续的监督和监测。在有足够细胞且保存良好的材料可用于电子显微镜检查的病例中，超微结构评估在48%的病例诊断中具有临床价值贡献。
