Tang C S, Kung I T
Institute of Pathology, Queen Elizabeth Hospital, Kowloon, Hong Kong.
Acta Cytol. 1993 Sep-Oct;37(5):689-93.
Homogenization of sputum with dithiothreitol (DTT) has not been used in cytology for the detection of malignant cells. We examined the efficacy of this method as compared to the conventional "pick and smear" (PS) method. Smears were prepared from sputum samples using the PS technique to act as the control. The remaining sputum was digested with an equal volume of 0.3% DTT in 70% ethanol at room temperature. The cells were washed with 35% ethanol and smears prepared. The DTT-treated specimens gave a much higher concentration of evenly distributed cells. The background was clear and the morphology comparable to that of the control. More positive cases were detected with the DTT than the conventional PS technique. Homogenization of sputum with DTT is a simple and effective method of screening for pulmonary malignancies.
用二硫苏糖醇（DTT）对痰液进行均质化处理尚未用于细胞学中恶性细胞的检测。我们将这种方法与传统的“挑取涂片”（PS）方法相比较，检验了其效果。使用PS技术从痰液样本制备涂片作为对照。将剩余痰液在室温下用等体积含0.3% DTT的70%乙醇消化。细胞用35%乙醇洗涤后制备涂片。经DTT处理的标本产生的细胞浓度更高且分布均匀。背景清晰，形态与对照相当。与传统PS技术相比，用DTT检测到的阳性病例更多。用DTT对痰液进行均质化处理是一种简单有效的肺部恶性肿瘤筛查方法。
