Hoorens A, Lemoine N R, McLellan E, Morohoshi T, Kamisawa T, Heitz P U, Stamm B, Rüschoff J, Wiedenmann B, Klöppel G
Department of Pathology, Academic Hospital Jette, Free University of Brussels, Belgium.
Am J Pathol. 1993 Sep;143(3):685-98.
In a series of 22 pancreatic acinar cell carcinomas, including two acinar cystadenocarcinomas, cellular differentiation was analyzed by immunocytochemistry and electron microscopy. In addition, overexpression of p53 protein and Ki-ras codon 12 mutation was studied. Four of the 20 noncystic acinar cell carcinomas showed a pure acinar pattern, nine an acinar-solid, and seven a solid pattern. All tumors stained for at least one of the following pancreatic acinar markers: trypsin (21 of 22), lipase (19 of 22), chymotrypsin (13 of 22), phospholipase A2 (nine of 22), and pancreatic stone protein (19 of 22). One-third of the tumors expressed neuroendocrine markers (synaptophysin, eight of 22; chromogranin A, six of 21) and duct cell markers (CA19.9, nine of 21; B72.3, six of 21). Cellular coexpression of trypsin and synaptophysin was demonstrated in one tumor. Electron microscopy revealed zymogen granules (nine of nine). In only one of 16 tumors a Ki-ras mutation at codon 12 was found, whereas in none of 19 tumors could overexpression of p53 protein be demonstrated. The results suggest that acinar cell carcinomas show obvious capacity to differentiate into several directions, but nevertheless constitute an entity different from ductal adenocarcinomas or endocrine tumors.
在一组22例胰腺腺泡细胞癌（包括2例腺泡状囊腺癌）中，通过免疫细胞化学和电子显微镜分析细胞分化情况。此外，研究了p53蛋白的过表达和Ki-ras密码子12突变情况。20例非囊性腺泡细胞癌中，4例表现为纯腺泡型，9例为腺泡实性型，7例为实性型。所有肿瘤至少对以下一种胰腺腺泡标志物染色：胰蛋白酶（22例中的21例）、脂肪酶（22例中的19例）、糜蛋白酶（22例中的13例）、磷脂酶A2（22例中的9例）和胰石蛋白（22例中的19例）。三分之一的肿瘤表达神经内分泌标志物（突触素，22例中的8例；嗜铬粒蛋白A，21例中的6例）和导管细胞标志物（CA19.9，21例中的9例；B72.3，21例中的6例）。在1例肿瘤中证实了胰蛋白酶和突触素的细胞共表达。电子显微镜显示有酶原颗粒（9例中的9例）。在16例肿瘤中仅1例发现密码子12处的Ki-ras突变，而在19例肿瘤中均未证实p53蛋白过表达。结果表明，腺泡细胞癌具有明显的向多个方向分化的能力，但仍构成一种不同于导管腺癌或内分泌肿瘤的实体。
