Craven S E, Cox N A, Bailey J S, Stern N J, Meinersmann R J, Blankenship L C
Poultry Microbiological Safety Research Unit, R. B. Russell Agricultural Research Center, U.S. Department of Agriculture, Athens, Georgia 30613.
Avian Dis. 1993 Apr-Jun;37(2):339-48.
This study determined the ability of eight strains of Salmonella and their agarsubcultured variants to colonize the intestinal tract of broiler chicks. Nalidixic-acid (NAL)-resistant and streptomycin-resistant subcultured strains (S. california 1989/A and S. typhimurium 3366/A) that persisted in the ceca of chicks in lower numbers than their NAL-resistant parent strains (1989/O and 3366/O) were selected for additional study S. typhimurium strain 3366/A was present in the ceca of chicks in lower numbers than the parent strain 3366/O when given concomitantly with the parent strain or when the two strains were given separately to different chicks. S. california 1989/A strain was present in the ceca in lower numbers than the parent strain after concomitant oral or intracloacal inoculation. Strains 3366/O and 3366/A of S. typhimurium differed in growth rates in BHI broth and cecal mucus. The lipopolysaccharide (LPS) profile indicated that LPS components present in S. california 1989/O were missing from strain 1989/A. A mutant of 1989/O--2095/R--was also LPS- and colonization-deficient.
本研究确定了8株沙门氏菌及其经琼脂传代培养的变体在肉鸡肠道内定殖的能力。选择了耐萘啶酸（NAL）和耐链霉素的传代培养菌株（加利福尼亚沙门氏菌1989/A和鼠伤寒沙门氏菌3366/A），它们在雏鸡盲肠中存活的数量低于其耐NAL的亲本菌株（1989/O和3366/O），以便进行进一步研究。当与亲本菌株同时给予雏鸡或分别给予不同雏鸡时，鼠伤寒沙门氏菌菌株3366/A在雏鸡盲肠中的数量低于亲本菌株3366/O。口服或经泄殖腔接种后，加利福尼亚沙门氏菌1989/A菌株在盲肠中的数量低于亲本菌株。鼠伤寒沙门氏菌的3366/O和3366/A菌株在脑心浸液肉汤和盲肠黏液中的生长速率不同。脂多糖（LPS）图谱表明，加利福尼亚沙门氏菌1989/O中存在的LPS成分在1989/A菌株中缺失。1989/O的一个突变体——2095/R——也存在LPS缺陷和定殖缺陷。
