Waterfield C J, Mesquita M, Parnham P, Timbrell J A
Toxicology Department, School of Pharmacy, University of London, Brunswick Square, U.K.
Biochem Pharmacol. 1993 Aug 17;46(4):589-95. doi: 10.1016/0006-2952(93)90542-5.
Exposure of rat hepatocytes to hydrazine, carbon tetrachloride or 1,4-naphthoquinone results in cytotoxicity determined as uptake of Trypan blue and leakage of lactate dehydrogenase (LDH). After exposure of hepatocytes to hydrazine and 1,4-naphthoquinone, ATP was also measured and was found to be depleted. Addition of the beta-amino acid taurine to the hepatocyte incubation buffer partially protects the cells against the cytotoxicity of these three different cytotoxic compounds, as indicated by Trypan blue uptake and LDH leakage. Taurine also reduces the depletion of ATP caused by 1,4-naphthoquinone but not hydrazine. It is suggested that taurine may have a cytoprotective effect in vitro and may be a useful tool for the investigation of mechanisms of cytotoxicity.
将大鼠肝细胞暴露于肼、四氯化碳或1,4-萘醌会导致细胞毒性，其通过台盼蓝摄取和乳酸脱氢酶（LDH）泄漏来确定。在肝细胞暴露于肼和1,4-萘醌后，还测量了ATP，发现其减少。向肝细胞孵育缓冲液中添加β-氨基酸牛磺酸可部分保护细胞免受这三种不同细胞毒性化合物的细胞毒性影响，这通过台盼蓝摄取和LDH泄漏得以体现。牛磺酸还可减少由1,4-萘醌而非肼引起的ATP消耗。提示牛磺酸在体外可能具有细胞保护作用，并且可能是研究细胞毒性机制的有用工具。
