Waterfield C J, Mesquita M, Parnham P, Timbrell J A
Department of Toxicology, School of Pharmacy, University of London, Brunswick Square, London WC1N 1AX, UK.
Toxicol In Vitro. 1994 Aug;8(4):573-5. doi: 10.1016/0887-2333(94)90019-1.
This study examined the protection of isolated rat hepatocytes against carbon tetrachloride, hydrazine and 1,4-naphthoquinone (1,4-NQ) toxicity. Hepatocytes were incubated with various concentrations of toxicant in the presence and absence of taurine (0-15 mm). The presence of taurine significantly decreased the cytotoxicity of each compound as measured by trypan blue uptake and lactate dehydrogenase leakage. The protection was related to the concentration of taurine, with a significant effect at 10 mm for all three compounds. When ATP was measured, however, taurine failed to protect against the depletion caused by hydrazine, whereas depletion due to 1,4-NQ was significantly ameliorated. The results suggest that taurine may protect cells from cytotoxicity as reflected by membrane damage but biochemical events underlying the toxicity, such as ATP depletion, may not be affected. Taurine may be a useful tool for the investigation of mechanisms of cytotoxicity.
本研究检测了分离的大鼠肝细胞对四氯化碳、肼和1,4-萘醌（1,4-NQ）毒性的防护作用。肝细胞在有和没有牛磺酸（0 - 15 mM）存在的情况下，与不同浓度的毒物一起孵育。通过台盼蓝摄取和乳酸脱氢酶泄漏测定，牛磺酸的存在显著降低了每种化合物的细胞毒性。这种保护作用与牛磺酸的浓度有关，对于所有三种化合物，10 mM时具有显著效果。然而，当测量ATP时，牛磺酸未能防止肼引起的ATP消耗，而1,4-NQ引起的消耗则得到显著改善。结果表明，牛磺酸可能保护细胞免受膜损伤所反映的细胞毒性，但毒性背后的生化事件，如ATP消耗，可能不受影响。牛磺酸可能是研究细胞毒性机制的有用工具。
