Lü Z Q, Ma J X, Zhou C W, Wang Y F, Wang J, Xiang J Y, Gao Y, Zeng X L
Xinjiang Institute of Chemistry, Academia Sinica, Urumqi, PRC.
Sci China B. 1993 Jun;36(6):693-701.
Five hybridomas stably secreting monoclonal antibodies (McAbs) to androstenedione were prepared by using artificially synthesized androstenedion-11 alpha-succinyl conjugate with bovine serum albumin (BSA) as antigen. These McAbs showed slightly high cross-reactivity with testosterone (8.1-12.3%) and estrone (0.8-2.5%) and high affinities ranging from 2.0 x 10(7) to 2.8 x 10(8) L/M. They were all of the IgG1 subclass. Xinjiang finewool ewes which were passively immunized with McAbs had higher circulating levels of progesterone (P) and luteinizing hormone (LH) than those of control ewes in two oestrous cycles and these changes led to increased ovulation rate and twin lambs born in young ewes.
以人工合成的雄烯二酮-11α-琥珀酰牛血清白蛋白（BSA）偶联物为抗原，制备了5株稳定分泌抗雄烯二酮单克隆抗体（McAbs）的杂交瘤细胞株。这些单克隆抗体与睾酮（8.1-12.3%）和雌酮（0.8-2.5%）有稍高的交叉反应性，亲和力较高，范围为2.0×10(7)至2.8×10(8) L/M。它们均为IgG1亚类。用单克隆抗体被动免疫的新疆细毛母羊在两个发情周期中，其循环孕酮（P）和促黄体生成素（LH）水平高于对照母羊，这些变化导致年轻母羊排卵率增加和产双羔。
