Gavrilov V B, Konev S V, Orekhova T A, Gorilenko A Ia
Biofizika. 1993 Jul-Aug;38(4):644-8.
The nile red fluorescence in solutions containing Triton X-100, human serum albumin and hexadecane in different combinations was investigated. The dye fluorescence in phosphate buffer after 2-4 min incubation strongly decreased because of the dye aggregation and was stable in a detergent solution. The intensity of nile red fluorescence in the detergent solution was not changed after the addition of equal weight concentration of albumin and increased more than 2 times after the addition of the same concentration of hexadecane. The detergent eliminated the nile red sorption by cuvette surface. It is concluded that the detergent addition increases nile red selectivity as a lipid probe in the lipid-protein systems.
研究了含有不同组合的 Triton X - 100、人血清白蛋白和十六烷的溶液中的尼罗红荧光。在磷酸盐缓冲液中孵育 2 - 4 分钟后，由于染料聚集，染料荧光强烈降低，而在洗涤剂溶液中则稳定。加入等重量浓度的白蛋白后，洗涤剂溶液中尼罗红荧光强度不变，加入相同浓度的十六烷后，荧光强度增加超过 2 倍。洗涤剂消除了比色皿表面对尼罗红的吸附。得出的结论是，添加洗涤剂可提高尼罗红作为脂质 - 蛋白质系统中脂质探针的选择性。
