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[用粪卟啉I修饰的IgG以及直接测量抗原-抗体复合物的可能性]

[IgG, modified with coproporphyrin I and the possibility of direct measurement of the antigen-antibody complex].

作者信息

Lepesheva G I, Turko I V, Shestakov V L, Chashchin V L

出版信息

Biokhimiia. 1993 Jun;58(6):938-43.

PMID:8364116
Abstract

Modification of rabbit IgG by coproporphyrin I activated by Woodward's reagent K as well as antigen-binding and fluorescent properties of coproporphyrin-IgG have been studied. It was shown that coproporphyrin I modified IgG retains its capacity to bind the antigen. The formation of the immune antigen-antibody complex increases the intensity of coproporphyrin-IgG fluorescence.

摘要

研究了用伍德沃德试剂K活化的粪卟啉I对兔免疫球蛋白G(IgG)的修饰作用以及粪卟啉-IgG的抗原结合和荧光特性。结果表明,经粪卟啉I修饰的IgG仍保留其结合抗原的能力。免疫抗原-抗体复合物的形成增强了粪卟啉-IgG的荧光强度。

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