利用10吉赫兹频域荧光测定法对[亮氨酸5]脑啡肽酪氨酸荧光的强度和各向异性衰减情况进行研究。

Intensity and anisotropy decays of [Leu5] enkephalin tyrosyl fluorescence by 10 GHz frequency-domain fluorometry.

作者信息

Lakowicz J R, Gryczynski I, Laczko G, Wiczk W

机构信息

University of Maryland School of Medicine, Department of Biological Chemistry, Baltimore 21201.

出版信息

Biophys Chem. 1993 Jul;47(1):33-40. doi: 10.1016/0301-4622(93)80030-m.

DOI:10.1016/0301-4622(93)80030-m

PMID:8364147

Abstract

The technique of 10 GHz frequency-domain fluorometry was used to resolve the complex picosecond intensity and anisotropy decays of the tyrosyl emission of [Leu5] enkephalin. Enhanced resolution of anisotropy decay was obtained by using acrylamide quenching of the tyrosyl fluorescence and global analysis of the frequency-domain anisotropy data obtained with different amounts of acrylamide. The data indicates a 44 ps correlation time for local tyrosine motions, and a 219 ps correlation time for overall rotational diffusion of the pentapeptide. Our data are consistent with an initial loss of fluorescence anisotropy from r0 = 0.4 to a value of r0 = 0.326 occurring during the first two picoseconds after excitation.

摘要

采用10 GHz频域荧光测定技术解析了[亮氨酸5]脑啡肽酪氨酸发射的复杂皮秒强度和各向异性衰减。通过对酪氨酸荧光进行丙烯酰胺猝灭以及对不同丙烯酰胺用量下获得的频域各向异性数据进行整体分析，提高了各向异性衰减的分辨率。数据表明，局部酪氨酸运动的相关时间为44皮秒，五肽整体旋转扩散的相关时间为219皮秒。我们的数据与激发后最初两个皮秒内荧光各向异性从r0 = 0.4初始衰减至r0 = 0.326的值相符。