Yagi T
Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037.
Biochem Mol Biol Int. 1993 Jun;30(2):253-60.
The M(r) 30,000 polypeptide of the hydrophobic protein fraction of the energy-transducing NADH-ubiquinone oxidoreductase (complex I) of bovine heart mitochondria was identified as the ND2 gene product based on a comparison of amino acid analysis and partial N-terminal sequencing results with the known DNA sequence of ND2 (Anderson, S. et al. (1982) J. Mol. Biol. 156, 683-717). A simple purification procedure was devised for this ND2 gene product. The procedure, which is described, involves treatment of bovine complex I with a chloroform-methanol (2:1 [v/v]) solution. The antiserum raised against this purified bovine ND2 gene product cross-reacted with the approximately M(r) 39,000 polypeptide extracted from the Paracoccus denitrificans membranes with chloroform-methanol (2:1 [v/v]).
基于对氨基酸分析和部分N端测序结果与已知ND2基因序列（Anderson, S.等人，(1982) J. Mol. Biol. 156, 683 - 717）的比较，牛心线粒体能量转换型NADH - 泛醌氧化还原酶（复合体I）疏水蛋白组分中分子量为30,000的多肽被鉴定为ND2基因产物。针对该ND2基因产物设计了一种简单的纯化方法。所描述的该方法包括用氯仿 - 甲醇（2:1 [v/v]）溶液处理牛复合体I。用这种纯化的牛ND2基因产物制备的抗血清与用氯仿 - 甲醇（2:1 [v/v]）从反硝化副球菌膜中提取的分子量约为39,000的多肽发生交叉反应。
