Galanis M, Wang L, Nagley P, Devenish R J
Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
Biochem Mol Biol Int. 1993 Jun;30(2):271-82.
Tandem duplication of a mitochondrial import leader sequence has been shown to markedly increase the efficiency of translocation of chimaeric precursors across mitochondrial membranes to the mitochondrial matrix. The principle of leader sequence duplication was applied to the protein secretion system of the yeast Saccharomyces cerevisiae and of Bacillus subtilis. The secretion signal sequences of yeast invertase and B. subtilis neutral protease were used to direct the secretion of human interferon alpha 4. Our results show that the duplication of these N-terminal signal sequences does not enhance secretion of interferon alpha 4 in either of the cell systems studied.
线粒体导入前导序列的串联重复已被证明可显著提高嵌合前体跨线粒体膜转运至线粒体基质的效率。前导序列重复的原理被应用于酿酒酵母和枯草芽孢杆菌的蛋白质分泌系统。利用酵母蔗糖酶和枯草芽孢杆菌中性蛋白酶的分泌信号序列来指导人α4干扰素的分泌。我们的结果表明，在上述所研究的两种细胞系统中，这些N端信号序列的重复均未增强α4干扰素的分泌。
