Comparative studies of intracellular activity, secretion and multiple forms spectra of human skin fibroblast alpha-L-fucosidase in the normaland after sucrose load.

Activity and multiple forms of intracellular and secreted alpha-L-fucosidases from human skin fibroblasts were studied in normal and sucrose-loaded cells--modelling intralysosomal nonhydrolyzable products' accumulation in vitro. Certain differences in secretion levels were found after sucrose load for alpha-L-fucosidases from embryonal and postnatal cell lines. Different effects of sucrose load on multiple forms' spectra of the intracellular and secreted alpha-L-fucosidases from embryonal and postnatal fibroblasts were revealed.