Parfett C L, Pilon R
Mutagenesis Section, Environmental Health Directorate, Health and Welfare Canada, Tunney's Pasture, Ottawa, Ontario.
Cancer Lett. 1993 Jul 30;71(1-3):167-76. doi: 10.1016/0304-3835(93)90112-m.
Transcripts from the murine gene family proliferin, which are increased by a wide assortment of chemical promoters of C3H10T1/2 cell morphological transformation, were shown to be induced by tri-n-butyltin chloride at concentrations above 50 nM. Two-stage transformation assays, with 3-methylcholanthrene as inducer and tri-n-butyltin chloride as promoter, were performed to determine if promotion of morphological transformation and proliferin induction were properties shared by this compound. Tri-n-butyltin chloride synergistically enhanced focus formation at concentrations ranging from 20 to 75 nM. Di-n-butyltin dichloride, n-butyltin trichloride and tin (II) chloride, but not tin (IV) chloride, were also effective inducers of proliferin. Changes in patterns of TPA-inducible, secreted proteins, including those likely to be proliferin, were detected following organotin treatment of confluent monolayers. Tri-n-butyltin chloride resembles other agents active as promoters in C3H10T1/2 two-stage transformation assays by possessing an ability to induce proliferin expression.
在C3H10T1/2细胞形态转化的多种化学启动剂作用下会增加的小鼠基因家族增殖蛋白的转录本，已表明在浓度高于50 nM时会被三丁基氯化锡诱导。进行了以3-甲基胆蒽为诱导剂、三丁基氯化锡为启动剂的两阶段转化试验，以确定形态转化促进作用和增殖蛋白诱导作用是否为此化合物所共有的特性。三丁基氯化锡在20至75 nM的浓度范围内协同增强了集落形成。二丁基二氯化锡、正丁基三氯化锡和氯化亚锡（而非四氯化锡）也是增殖蛋白的有效诱导剂。在用有机锡处理汇合的单层细胞后，检测到了佛波酯诱导的分泌蛋白模式的变化，包括那些可能是增殖蛋白的蛋白。三丁基氯化锡与在C3H10T1/2两阶段转化试验中作为启动剂起作用的其他试剂类似，具有诱导增殖蛋白表达的能力。
