Takeshita M, Grollman A P, Ohtsubo E, Ohtsubo H
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5983-7. doi: 10.1073/pnas.75.12.5983.
The sequence of oligonucleotides produced by the action of bleomycin and ferrous ion on double- and single-stranded DNA has been determined. In the presence of ferrous ion, bleomycin promotes cleavage at G-T and G-C sequences, while high concentrations of ferrous ion alone result in strand scission that is not base specific. In the presence of bleomycin and ferrous ion, pyrimidine bases located to the 3' side of guanosine are released preferentially and a low molecular weight product that forms a chromophore with thiobarbituric acid is produced from the deoxyribose moiety. Oligonucleotides produced by the action of bleomycin differ slightly in electrophoretic mobility from those produced by chemical or enzymatic cleavage. A model is proposed to explain the interactions of bleomycin with DNA.
已经确定了博来霉素和亚铁离子作用于双链和单链DNA所产生的寡核苷酸序列。在亚铁离子存在的情况下,博来霉素促进在G-T和G-C序列处的切割,而单独的高浓度亚铁离子会导致不具有碱基特异性的链断裂。在博来霉素和亚铁离子存在的情况下,位于鸟苷3'侧的嘧啶碱基优先释放,并且从脱氧核糖部分产生一种与硫代巴比妥酸形成发色团的低分子量产物。博来霉素作用产生的寡核苷酸在电泳迁移率上与化学或酶促切割产生的寡核苷酸略有不同。提出了一个模型来解释博来霉素与DNA的相互作用。